Fig. S2.

Histological analysis of human CRC zebrafish xenografts. Hematoxylin eosin staining (A–E′) and immunohistochemistry for CK20 (F–J′) were performed in histological sections, and corresponding images were obtained using a Zeiss AxioScan Z1, generating tiled images. Note that a fine line of agarose inclusion might be detected around the xenograft due to the agarose embedding step prior to paraffin inclusion. Whole-mount imunofluorescence staining for laminin (K–O) and β-catenin (P–T′). All imunofluorescence images are at the same magnification. (Scale bar, 50 μm.) DiI is in red, and nuclei staining with DAPI is in blue. Dashed boxes indicate areas of zoom. All images are anterior to the left, posterior to right, dorsal up, and ventral down.