Fig. 4.

Simultaneous measurements of shape fluctuations of CGS and NE upon biochemical perturbations. (A) Micrographs of cell nuclei expressing both H2B-mCherry (green) and LMNA-GFP (red) under the following conditions: control, after ATP depletion, and upon addition of $\alpha$-amanitin, blebbistatin, latrunculin A, and nocodazole. (B) Fluctuations $u^2$ of CGS (green) and NE (red) for each condition from A at one time point. Average and instantaneous contours for CGS and NE are shown in Fig. S6. (C) Wavenumber-dependent $⟨u_q^2⟩$ under the following conditions: control (n = 27), ATP depletion (n = 24), $\alpha$-amanitin (n = 15), blebbistatin (n = 15), latrunculin A (n = 16), and nocodazole (n = 18) treatment. Black curves represent negative control by fixation with formaldehyde for CGS (markers) and NE (no markers). Solid black lines illustrate slopes $\sim q^{-2}$ and $\sim q^{-4}$. Error bars are shown in Fig. S6. (D) PSD of $⟨u_q^2⟩$ as a function of wavelength $\lambda$ upon different perturbations. (Scale bar, 5 μm.)