Fig. S1.

In vivo measurements of the shape fluctuations of the CGS and NE. (A) Fluctuations $u$ calculated for the contours of CGS from Fig. 1B at $t$ = 5, 8, and 13 s. (B) Fluctuations $u^2$ calculated for the contours of CGS from Fig. 1B at $t$ = 5, 8, and 13 s. (C) Fluctuations $u$ calculated for the NE contours from Fig. 1E at $t$ = 5, 13, and 23 s. (D) Fluctuations $u^2$ calculated for the NE contours from Fig. 1E at $t$ = 5, 13, and 23 s. In addition, in A–D, we plot $u$ and $u^2$ for samples fixed with formaldehyde (black), demonstrating that our measurements are well above the noise floor.