Fig. S5.

Analysis of fluctuation sites upon different perturbations. (A) Histogram of ratio of fluctuation sites $r_f$ for each condition. Ratio of fluctuation sites was determined by counting the sites with fluctuation ($u^2>0$) along the nuclear contour and normalized by its length. Green bars represent the fluctuations for CGS, where $u_{cgs}^2>0$, and red bars represent the fluctuations for NE, where $u_{ne}^2>0$. (B) Histograms of fluctuation rate $k_f$ at each condition. The rate of a fluctuation site was calculated by counting the time points with fluctuations $u^2>0$ at that fluctuation site and normalized by the length of measurement (20 s). (C) Histogram of fluctuation duration ${\tau }_f$ for every condition; ${\tau }_{f,1}$ shows the region of short duration, and ${\tau }_{f,2}$ shows the region of long duration. (D) Histogram of ratio of separation sites $r_{s,tot}$ for each condition. Ratio of separation sites was determined by counting the sites with separation ${(u_{cgs}-u_{ne})}^2\ne 0$ along the nuclear contour and normalized by its length. (E) Histogram of the fraction of separation sites $r_{s,f}$ distribution calculated by normalizing data from D by the average value of $r_f$ obtained from A for each condition. (F) Histogram of separation rate $k_s$ at each condition. The rate of a separation site was calculated by counting the time points with separation at that separation site and normalized by the length of measurement (20 s). (G) Histogram of separation duration ${\tau }_s$ for every condition; ${\tau }_{s,1}$ shows the region of short duration, and ${\tau }_{s,2}$ shows the region of long duration. Green bars represent ${\tau }_{s,\hspace{0.17em}2,cgs}$ for $(u_{ne}^2-u_{cgs}^2)<0$, and red bars represent the ${\tau }_{s,\hspace{0.17em}2,ne}$ for $(u_{ne}^2-u_{cgs}^2)>0$.