Table S1.

Summary of fluctuation characteristics upon biochemical perturbations

Condition	$⟨u_q^2⟩$	$r_f$	$k_f$	${\tau }_{f,\hspace{0.17em}1}$	${\tau }_{f,\hspace{0.17em}2}$	$r_{s,tot}$*	$r_{s,f}$ †	$k_s$	${\tau }_{s,\hspace{0.17em}1}$	${\tau }_{s,\hspace{0.17em}2,cgs}$	${\tau }_{s,\hspace{0.17em}2,ne}$
Control	$⟨u_{q,control}^2⟩$	0.66 $\pm$ 0.04	0.22 $\pm$ 0.04	<1 s	$\sim$5 s	0.18 $\pm$ 0.05	0.28 $\pm$ 0.08	0.07 $\pm$ 0.02	<1 s	—	$\sim$5 s
ATP depletion	Decrease	0.53 $\pm$ 0.06	0.23 $\pm$ 0.04	<1 s	$\sim$5 s	0.16 $\pm$ 0.05	0.30 $\pm$ 0.10	0.06 $\pm$ 0.01	<1 s	—	—
$\alpha$-Amanitin	Increase	0.72 $\pm$ 0.06	0.25 $\pm$ 0.06	<1 s	$\sim$5 s	0.15 $\pm$ 0.03	0.21 $\pm$ 0.05	0.09 $\pm$ 0.04	<1 s	$\sim$6 s	$\sim$6 s
Blebbistatin	Decrease	0.61 $\pm$ 0.05	0.21 $\pm$ 0.01	<1 s	$\sim$5 s	0.20 $\pm$ 0.07	0.33 $\pm$ 0.11	0.08 $\pm$ 0.02	<1 s	$\sim$5 s	—
Latrunculin A	Increase	0.92 $\pm$ 0.04	0.41 $\pm$ 0.06	<1 s	$\sim$6 s	0.39 $\pm$ 0.06	0.43 $\pm$ 0.07	0.09 $\pm$ 0.03	<1 s	$\sim$5 s	—
Nocodazole	Decrease	0.38 $\pm$ 0.07	0.17 $\pm$ 0.04	<1 s	$\sim$5 s	0.13 $\pm$ 0.07	0.33 $\pm$ 0.19	0.08 $\pm$ 0.02	<1 s	$\sim$5 s	—

^*Ratio of the number of separation sites to the length of contour.

^†Ratio of the number of separation sites to the number of fluctuating sites.