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. 2017 Sep 12;114(39):E8274–E8283. doi: 10.1073/pnas.1703420114

Fig. S2.

Fig. S2.

Ch, D2, and GFP fluorescent proteins affect the survival of cortical neurons distinctly. (A) Cumulative risk estimates of primary neurons transfected with Ch, D2, and GFP (D2 = 386, GFP = 210, and Ch = 141 neurons, respectively; log-rank test; n.s., nonsignificant, ***P < 0.001). (B) Cohorts of neurons expressing similar levels of D2 and SynD2 were selected for further analysis of the risk of neuronal death (D2 = 119 and SynD2 = 125 neurons, respectively; Mann–Whitney test; n.s., nonsignificant). (C) The risk of death of cohorts of neurons expressing similar levels of WT aSyn (SynD2) and control protein D2 (from B) was compared (log-rank test; n.s., nonsignificant). (D) Cohorts of neurons expressing similar levels of GFP and SynGFP were selected (SynGFP = 132 and GFP = 210 neurons; Mann–Whitney test; n.s., nonsignificant). (E) The risk of death in cohorts of neurons was compared after expressing similar levels of WT aSyn (SynGFP) and control protein GFP (from D; log-rank test; n.s., nonsignificant). All error bars represent the 95% CIs.