Fig. S1.

(A) Bound intensity for long (90-min) and short (<20-min) nanoswitch construction times for 200 pM PSA. Hybridization conditions are described in Methods, and error bars are the SEM over four replicates. (B) Distribution of melting temperatures in the hybridization buffer (50 mM NaCl, 10 mM MgCl₂) for all 121 tiling oligonucleotides computed by the OligoAnalyzer Tool (IDT). (C) Measurement of annealing using SYBR Green I during a normal 90-min hybridization time course stepping down 1° per minute for only the M13 scaffold, only the tiling oligonucleotides, and both the M13 and tiling oligonucleotides.