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. 2017 Sep 6;114(39):E8214–E8223. doi: 10.1073/pnas.1710430114

Fig. 4.

Fig. 4.

Presence of sub-RPE deposits with drusen-like composition underneath aged (D90) SFD and DHRD hiPSC-RPE cultures. (A–D) Confocal images of age-matched (D90) Ctrl vs. SFD and DHRD hiPSC-RPE cross-sections displayed the presence of TIMP3-APOE–positive (A), EFEMP1-APOE–positive (B), CRYAA/CRYAB-APOE–positive (C), and APOE-positive deposits underlying basement membrane marked by COL4 (D) in SFD and DHRD hiPSC-RPE cultures. (Scale bar: 25 µm.) Of note, sporadic APOE-positive sub-RPE basal deposits (A and D) were observed underneath Ctrl hiPSC-RPE cultures. (E and F) Quantitative Western blot analyses revealed increased amount of COL4 protein in the ECM underlying SFD and DHRD hiPSC-RPE cultures compared with Ctrl hiPSC-RPE cultures at D90. Of note, data are presented as mean + SEM, and COL4 bands at ∼250, 150, and 70 kDa are consistent with multimeric and monomeric α1 and α2 subunits and fragments of human COL4 protein. *P ≤ 0.05.