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Characterization of the aggregates. The pSyn-immunostained aggregates (green) observed in the SN express features characteristic of Lewy bodies (arrowheads) and Lewy neurites (arrows), staining positively for ubiquitin (red) (A–C) and thioflavin (green in E), and being resistant to proteinase K (ProtK) digestion (D and D′). Images were captured from an α-SYN + FIB–treated animal at 3 wk post-PFF injection. (Scale bars: A–C, 50 μm; D, 100 μm; D′, 50 μm; E, 10 μm.)
