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. 2017 Sep 11;114(39):E8224–E8233. doi: 10.1073/pnas.1712176114

Fig. 5.

Fig. 5.

RABIF regulates the stability of Rab10. (A) Immunoblots showing the expression levels of endogenous Rab10 and α-tubulin in the indicated adipocytes. (B) Lentiviral expression of FLAG-tagged Rab10 in WT or Rabif-KO adipocytes. The levels of tagged Rab10 and endogenous α-tubulin were probed by immunoblotting. (C) Rabif-KO adipocytes were either untreated or treated with 10 µM MG132 or 100 nM PS341 for 24 h. The expression levels of endogenous Rab10 and α-tubulin were probed by immunoblotting. (D) WT and Rabif-KO adipocytes were treated with 10 µM MG132 or 100 nM PS341 for 24 h in the absence or presence of 100 ng/mL cycloheximide. The expression levels of endogenous Rab10 and α-tubulin were probed by immunoblotting. (E) WT or RABIF-KO HeLa cells were either untransfected or transiently transfected with cerulean-tagged Rab10 or Rab13. The expression levels of Rab10, Rab13, and α-tubulin were probed by immunoblotting. (F) Flow cytometry measurements of the surface levels of the GLUT4 reporter using cells from E. **P < 0.01. n.s., not significant. Error bars indicate SD.