Fig. S3.

Rab10 and RABIF interact with each other and partially colocalize in the cell. (A) RABIF-FLAG was transiently expressed in RABIF-KO HeLa cells. RABIF-FLAG was immunoprecipitated using anti-FLAG antibodies, and the presence of Rab10 in the precipitates was detected by immunoblotting. (B) Representative confocal images showing the localization of Rab10-Myc and RABIF-FLAG in HeLa cells with or without 30 min of insulin treatment (100 nM). (Scale bars: 10 µm.) (Magnification: B, Right, 40×.)