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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Jun 3;141(1):152–162. doi: 10.1016/j.jaci.2017.04.045

Figure 4. PGE2 exacerbates atopic skin inflammation in the repeated oxalozone (OXA) challenge model.

Figure 4

A-E, WT C57BL/6 mice were sensitized with 3% OXA on abdominal skin on (day 0) and then challenged with 0.6% OXA or EtOH on ears 5 days later. Challenge with OXA was repeated once every two days for a total of 5 challenges. Ears and ear-draining LNs were collected 6 h after the last OXA challenge (n=4-5). A, Schematic representation of the experimental protocol. B, Ear thickness. C, Ear histology. Scale bar, 50 μM. D, CD4+ and CD8+ T cells in ear-draining LNs. E, IL-22 production by ear-draining LN cells cultured with soluble anti-CD3 or medium only in vitro for 3 days. F, Swelling of ears from control and EP4cKO mice (n=9-13) sensitized and repeatedly challenged with OXA as in A but without indomethacin treatment. *P<0.05; ***P<0.001 and ****P<0.0001.