Figure 3.

The A. fumigatus ΔflcA has morphogenetic defects. The wild-type, ΔflcA-C, and their corresponding complementing strains were grown for 48 h at 37 °C on solid (A) or liquid MM (B). A. fumigatus wild-type and ΔflcA germlings were grown in liquid MM for 12 h and stained or not with calcofluor white (C, top panels, bars 5 µM) or for 20 h at 30 °C (C, lower panels, bars, 10 µM). (D) The edge of the colonies represented in the plates of (A). Bars, 50 µM. (E) The qRT-PCR for the A. fumigatus flcA-C genes in the wild-type, ΔflcA, ΔflcB, and ΔflcC strain. The strains were grown for 16 hours at 37 °C (time 0) and transferred to 200 mM CaCl₂ for 10 and 30 min. The results are expressed as the number of cDNA copies of a specific flc gene divided by the number of copies of the cDNA of the normalizer βtub (p < 0.001).