Figure 4.

The ΔflcA mutant strain is more sensitive to calcium stress, cell wall damaging and oxidative stressing agents. (A) The wild type, the mutant, and the complemented strains were grown on YAG medium with increasing concentrations of EGTA, cyclosporin, calcofluor white (CFW), congo red (CR), Sodium Dodecyl Sulfate (SDS), t-butyl hydroxyperoxide, and paraquat for 48 h at 37 °C. (B) Radial growth of the wild-type and ΔflcA mutant strains grown in YAG medium for 120 hours at 37 °C in the absence and presence of increasing CaCl₂ concentrations. The results are the average ± standard deviation of 3 repetitions. Statistical analysis was performed by using One-way Anova with post test Dunnett (*** p < 0.05).