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. 2016 Oct 20;8(6):848–858. doi: 10.1080/21505594.2016.1250994

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — qPCR based validation and corresponding secreted proteins of differentially expressed cytokines and chemokines in BMDM after M.tb infection. A. Relative mRNA expression (fold change) of various cytokines and chemokines induced by BMDMs following infection with hypo- and hypervirulent M.tb as analyzed through qPCR (n = 3). B. Corresponding heatmap as generated by RNAseq under the same infection conditions (Red-Upregulation, Green-downregulation). C and D. Corresponding secreted cytokines and chemokines in BMDMs under the same infection conditions as measured by ELISA (n = 6). The means and standard error of a minimum of 3 independent experiments are shown, * indicates significance p < 0.05. Legend corresponds to all 3 graphs (A, C and D).