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. 2016 Oct 20;8(6):848–858. doi: 10.1080/21505594.2016.1250994

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Virulence-specific gene expression confirmed through qPCR and Western blot in BMDM and THP-1 macrophages infected with hypo- and hypervirulent M.tb. A. Relative mRNA expression (fold change) of selected differentially expressed genes induced by BMDMs following infection with hypervirulent M.tb after 12 h of infection as analyzed through qPCR (n = 4). B. Corresponding heatmap as generated by RNAseq under the same infection conditions in BMDMs (Red-Upregulation, Green-downregulation), Rep = Replicate. C. Relative mRNA expression (fold change) of the same virulence-specific genes induced by THP-1s following infection with hypervirulent M.tb after 12 h of infection as analyzed through qPCR (n = 4). D. Western blot of corresponding proteins expressed by BMDMs and THP-1s under the same infection conditions, GAPDH was used as a loading control (n = 4), Uninf. = Uninfected BMDM and THP-1, Hypov.= Hypovirulent infection, Hyperv. = Hypervirulent infection. *p < 0.05 indicates significance, a minimum of 3 biological replicates were performed for each experiment.