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. 2016 Sep 19;8(6):751–766. doi: 10.1080/21505594.2016.1232239

Figure 7.

Figure 7.

Adoptive transfer. The C57BL/6 BM sell+/+selp+/+, sell−/−selp+/+ and sell+/+selp−/−Ly6C cells or BALB/c BM harboring 105 CFU of B. pseudomallei were used as donors (blue). As controls, the Ly6C cells were immediately mixed with 105 CFU of free B. pseudomallei (red). After adoptive transfer of sell+/+selp+/+, sell−/−selp+/+ and sell+/+selp−/−cells, the bacterial colonization of brains (A) and proportions of Ly6C+GFP+ cells (B) were determined for C57BL/6 recipients. For antibody blocking, the B. pseudomallei-loaded C57BL/6 or BALB/c BM Ly6C cells were pretreated with anti-CD62L antibody (CD62L), rabbit control IgGs (IgG) or bovine serum albumin (BSA) or were without treatment (W/O). After adoptive transfer, the bacterial colonization of brains in C57BL/6 and BALB/c recipients was respectively shown. The (*) symbol indicates p < 0.05 between lined groups (Mann-Whitney U test for Fig. 7A and 7B) or multiple groups (ANOVA and Tukey HSD test for Fig. 7C).