Table 1.

Primary antibodies used for flow cytometry or immunohistochemistry in this study.

Target	Representative to	Conjugateda	Isotype control	Clone	Species	Concentration	Company
CD11b,	Leukocytes	PE-Cy7, PE or none	IgG2b κ	M1/70	Rat	2 μg/mL	BD Pharmingen
Ly6C,	Monocytes	PE0Cy7, PE or none	IgM κ	AL-21	Rat	2 μg/mL	BD Pharmingen
Ly6G,	Neutrophils	PE or none	IgG2a κ	1A8	Rat	2 μg/mL	BD Pharmingen
F4/80,	Macrophages/Kupffer cells	PE or none	IgG2a κ	BM8	Rat	2 μg/mL	BioLegend
CD3e,	T cells	none	IgG1 κ	145-2C11	Hamster	20 μg/mL	BD Pharmingen
CD19	B cells	none	IgG2a κ	1D3	Rat	2 μg/mL	BD Pharmingen
Bpb		none	IgG1	polyc	Rat	30 μg/mL	Ref. (52)
CD45,	Leukocyte common antigens	PE	IgG2a κ	MEC13.3	Rat	2 μg/mL	BD Pharmingen
CD16/32	Phagocytic cells	PE-Cy	IgG2b κ	2.4G2	Rat	2 μg/mL	BD Pharmingen
CD34	Stem cells	PE	IgG2a κ	RAM34	Rat	2 μg/mL	BD Pharmingen
CD115	Phagocytic precursor	PE	IgG1 κ	T38-320	Rat	5 μg/mL	BD Pharmingen
CD14	Monocytes/macrophages	PE	IgG1 κ	rmC5-3	Rat	2 μg/mL	BD Pharmingen
CD150	Lymphoid cells	PE	IgG2a κ	Q38-480	Rat	2 μg/mL	BD Pharmingen
CD62L	BM inflamed cells	PE-Cy	IgG2a κ	MEL-14	Rat	4 μg/mL	BD Pharmingen
CD31	BM inflamed cells	PE	IgG2a κ	MEC 13.3	Rat	2 μg/mL	BD Pharmingen
CCR2	BM inflamed cells	PE	IgG2b	475301	Rat	2 μg/mL	R&D systems
CX3CR1	Resident cells in BM	PE	IgG2a κ	SA011F11	Mouse	5 μg/mL	BioLegend

Notes.

^a: PE: phycoerythrin; PE-Cy7: phycoerythrin-Cyanine 7

^b: Bp: Burkholderia pseudomallei

^c: poly: polyclonal antibodies