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. 2016 Oct 7;8(6):741–750. doi: 10.1080/21505594.2016.1231295

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Figure 3. — SEK binding competition between mAb combinations. A 96-well ELISA plate was coated with 25ng of SEK per well, blocked with 1% BSA, and then simultaneously treated with (A) the combination of mAb-4G3 (IgG2b) and mAb-5G2 (IgG1) or (B) the combination of mAb-9H2 (IgG1). MAb-4G3 and mAb-5G2 do no exhibit competition for epitope(s) on SEK. mAb-4G3 and mAb-9H2 exhibit competition for epitope(s) on SEK. All experiments were performed in duplicate. GAM = Goat anti-mouse, AP = Alkaline phosphatase.