Table 1.
Bacterial strains and plasmids.
| Strain | Relevant genotype and/or information | Source or reference |
|---|---|---|
| 12023 | Wild-type | Laboratory stock |
| PB7956 | 12023 - ΔfadBA | García-Véscovi |
| PB8099 | 12023 - ΔfadD | 68 |
| ΔfadD - pfadD | 12023 - ΔfadD - pfadD | This study |
| Plasmids | ||
| pFPV25 | GFP reporter fusion vector (ApR) | 42 |
| pMAN-3 | pFPV25 derivative carrying the fadAB::gfpmut3a promoter (ApR) | This study |
| pMAN-4 | pFPV25 derivative carrying the fadL::gfpmut3a promoter (ApR) | This study |
| pMAN-5 | pFPV25 derivative carrying the fadD::gfpmut3a promoter (ApR) | This study |
| pMAN-6 | pFPV25 derivative carrying the fadE::gfpmut3a promoter (ApR) | This study |
| pMAN-11 | pFPV25 derivative carrying the aceBA::gfpmut3a promoter (ApR) | This study |
| pMAN-12 | pFPV25 derivative carrying the pfkA::gfpmut3a promoter (ApR) | This study |
| pMAN-13 | pFPV25 derivative carrying the edd::gfpmut3a promoter (ApR) | This study |
| p28LL | pET28a(+) carrying gene fadD, as a His-tag fusión (KmR) | This study |