Table 2.
Oligonucleotides used in this study.
| Oligonucleotide | Sequence (5′ to 3′) | Restriction site |
|---|---|---|
| fadBAfw | CCCGGATCCCTCGCCAGAATGAATAAGTAACG | BamHI |
| fadBArev | CCCTCTAGAGTCAGTCTCCTGAATCCAC | XbaI |
| fadDfw | CCCGGATCCCAGGTCGTCATGCCTCCAC | BamHI |
| fadDrev | CCCTCTAGACACAACCCCAGTTAATAAAC | XbaI |
| fadLfw | CCCGGATCCGGCATTCGCCTCTCCAGTC | BamHI |
| fadLrev | CCCTCTAGAAACCTCATTGATTATTTTTATAC | XbaI |
| fadEfw | CCCGGATCCAAAAATTAGCCAGCGTTTCC | BamHI |
| fadErev | CCCTCTAGAAACGAAAAGCTCCCTTGC | XbaI |
| aceBAfw | CAGATGGATCCATATGATCTGCGTCACATG | BamHI |
| aceBArev | TGTGGTCTAGACATGCAGCTCCTCGTTGTTG | XbaI |
| pfkAup | GACTGGTACCATTCAGATTCATTTGG | KpnI |
| pfkAdn | TTCTTTCTAGAGACTACCTCTGAACTTTGG | XbaI |
| eddfw | GCGCTGGATCCCCGCCGTGACGAAGTGG | BamHI |
| eddrev | CCCCCTCTAGACATAGAGGCTCCTGAAA | XbaI |
| fadDup(P) | AGAGGTCATATGTTGAAGAAGGTTTGGCTTAAC | NdeI |
| fadDdn(P)1 | AGCTGGGATCCCGCTCAGGCTTTATTGTC | BamHI |