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. 2016 Nov 10;8(6):821–847. doi: 10.1080/21505594.2016.1258507

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — IglC and VgrG are secreted in response to KCl. Indicated F. novicida strains were grown in TSB with or without 5 % KCl and FPI protein synthesis (pellet fractions) and secretion (cleared culture supernatants) were analyzed using SDS-PAGE and immunoblotting with specific antiserum. The band highlighted with an asterisk corresponds to VgrG, while the lower band corresponds to an unspecific band recognized by the antiserum. The inner membrane protein PdpB was included as a lysis control. The experiment was repeated 3 times and a representative example is shown.