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. 2016 Nov 10;8(6):821–847. doi: 10.1080/21505594.2016.1258507

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 8. — Cytopathogenicity of F. tularensis IglE mutant strains, including (A) lipobox mutants, (B) frameshift mutants, and (C) substitution mutants within the region overlapping with FS4. Culture supernatants of infected J774 cells were assayed for LDH activity at 0, 24 and 48 h and the activity was expressed as a percentage of the level of non-infected lysed cells (positive lysis control). Means and SD of triplicate wells from one representative experiment of 2 are shown. The asterisks indicate that the cytopathogenicity levels were significantly different from LVS-infected cells at a given time point as determined by a 2-sided t-test with equal variance, including the Bonferroni correction for multiple pair-wise comparisons (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001).