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. 2016 Nov 10;8(6):821–847. doi: 10.1080/21505594.2016.1258507

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 10. — IglC secretion promoted by F. novicida ΔiglE expressing various IglE mutants in trans, including (A) lipobox- and frameshift mutants, (B) deletion mutants, and (C) substitution mutants within the region overlapping with FS4. Indicated F. novicida strains were grown in TSB with 5 % KCl and FPI protein synthesis (pellet fractions) and secretion (cleared culture supernatants) were analyzed using SDS-PAGE and immunoblotting with anti-IglC antiserum. Immunoblotting for the inner membrane protein PdpB was included as a lysis control. The experiment was repeated 3 times and a representative example is shown.