Figure 11.

Secretion of IglE and the mutant variant FS1 into J774 macrophages. Macrophages were infected with LVS, ΔiglG or the F. novicida bla mutant FTN1072 expressing TEM fusions of wild-type IglE (WT) or the frameshift mutant FS1, carrying altered sequence of codons 2 to 6. After infection, cells were washed and loaded with CCF2/AM and analyzed using live cell microscopy. TEM β-lactamase activity is revealed by the blue fluorescence emitted by the cleaved CCF2 product, whereas uncleaved CCF2 emits a green fluorescence.