Figure 1.

Reactivity of five mAbs with native and O-deacetylated CP5 and CP8. (A) Structural similarities between CP5 and CP8 and their sites of O-acetylation (O-Ac). Both CP5-specific mAbs (4C2 and 5D1) reacted with (B) native CP5, whereas only mAb 4C2 reacted with (C) O-deacetylated CP5 (De-O-CP5). Three CP8-specific mAbs (5A6, 3B5, and 4G5) reacted with (D) native CP8, whereas only 5A6 and 3B5 reacted with (E) O-deacetylated (De-O-CP8) CP8. D-ManNAcA, N-acetyl-D-mannosaminuronic acid; L-FucNAc, N-acetyl-L-fucosamine; D-FucNAc, N-acetyl-D-fucosamine. Each sample was tested in duplicate, and the ELISAs were performed at least twice. The mean values of a representative experiment are shown.