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. 2016 Sep 21;8(6):767–781. doi: 10.1080/21505594.2016.1237333

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Toxin ELISAs. A. The C. DIFFICILE TOX A/B II™ ELISA assay kit from TechLab was used to measure combined toxin A and B in strains 630, 630Δerm, 630Δerm A+B-, 630E, 630E A+B-, 630E A+B- CT, 630E_topA, 630E_flgB and 630E_CD2667 grown in TY for 72 h. B. and C. Toxin ELISAs TGCbiomics, measuring the toxins separately were used to quantify toxin A (B) and toxin B (C) produced by strains 630, 630Δerm, 630Δerm A+B-, 630E, 630E A+B- and 630E A+B- CT grown in TY for 72 h. Statistics were performed using one-way ANOVA with Fisher's LSD test.