Table 2.
Strains and plasmids used in this study.
| Name | ||
|---|---|---|
| Bacterial strains | Description | Source |
| E. coli TOP 10 | F– mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ (ara leu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| E. coli CA434 | Conjugation donor | Williams et al.36 |
| C. difficile 630 | Wild-type | Brendan Wren |
| C. difficile 630Δerm | Erythromycin sensitive strain of C. difficile 630 | Hussain et al.13 |
| C. difficile 630Δerm(ΔpyrE) | C. difficile 630Δerm containing a deletion in the pyrE gene | Ng et al.19 |
| C. difficile 630E | Erythromycin sensitive strain of C. difficile 630 | Lyras et al.8 |
| C. difficile 630Δerm A+B- | C. difficile 630Δerm tcdB-1511a::intron ermB | Kuehne et al.9 |
| C. difficile 630E A+B- | C. difficile 630E | Lyras et al.8 |
| C. difficile 630E A+B-CT | C. difficile 630E tcdB-1511a:: intron ermB | This study |
| C. difficile 630E_topA | C. difficile 630E | This study |
| C. difficile 630E_flgB | C. difficile 630E | This study |
| C. difficile 630E_CD2667 | C. difficile 630E | This study |
| Plasmids | ||
| pMTL007C-E2:tcdB-1511a | ClosTron plasmid containing retargeted region to tcdB at IS 1511 (antisense oriented) for C. difficile 630Δerm or 630E | Kuehne et al.9 |
| pMTL-SC7315λ2.3::topA | pMTL-SC7315λ2.3 containing 1,000 bp homology arms to change nucleotide 1480649 from T to C in 630E | This study, based on Cartman et al.15 |
| pMTL-SC7315λ2.3::flgB | pMTL-SC7315λ2.3 containing 1,156 bp homology arms to reverse the inversion upstream of flgB in 630E | This study, based on Cartman et al.15 |
| pMTL-SC7315λ2.3::CD2667 | pMTL-SC7315λ2.3 containing 1,000 bp homology arms to change nucleotide 3079815 from C to A in 630E | This study, based on Cartman et al.15 |