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Virulence gene expression during intracellular survival. Differential expression of virulence genes (Table 2) relative to basal expression in culture medium was determined after 1 hr (A) and 24 hr (B) intracellular survival. Dashed lines mark 2-fold change in expression. Data shown are representative experiments of 3–4 biological replicates performed in triplicate. (**P < 0.01, ***P < 0.001, ****P < 0.0001).
