Figure 7.

PPARγ is largely dispensable for lung DC activation and antigen uptake but mediates antigen transport by CD11b⁺ DCs to the dLN. WT and AM-reconstituted Cd11c-CrePparg^fl/fl were injected intratracheally with 100 µg OVA-AF488 and 100 µg HDM and sacrificed 24 h later for analysis of lung and dLNs by flow cytometry. DC subsets were identified as CD45⁺ Siglec-F⁻CD11c⁺MHCII^high for the lung and CD45⁺autofluorescent⁻CD11c⁺MHCII^high for the lung and dLNs, respectively. Frequency of OVA-AF488⁺ among each DC subset in the lung dLN (A) and the total number of OVA-AF488⁺ DCs (B). Frequency of OVA-AF488⁺ among each DC subset in the lung (C) and the total the total number of OVA-AF488⁺ DCs (D). MFIs of indicated DC activation markers for lung dLN (E–G) and lung (H–J) DC subsets as a summary of FACS data. (K and L) MFI of ST2-expressing cells among DCs in the lung (K) and the lung dLN (L; n = 4–6/group). The data are representative of two experiments and are means ± SEM. The Student’s t test (unpaired) was used. *, P < 0.05.