Figure 3.

ZIKV infects isolated human glioblastoma but not normal brain tissue slices. (A–C) Representative images from three independent experiments showing freshly resected glioblastoma after staining with H&E (A) or for Ki-67 (B) or GFAP (C). (D–R) Immunofluorescence staining of glioblastoma tissue uninfected (D–F) or infected with ZIKV-Dakar (G–L) or ZIKV-Brazil (M–R) after 7 d for ZIKV E protein (green) and DAPI (blue) with Sox2 (red; D, G, J, M, and P), Ki-67 (red; E, H, K, N, and Q), or GFAP (red; F, I, L, O, and R). D–R are representative images from three independent experiments. (S) Quantification of tumor cells infected with ZIKV (left) and ZIKV-infected cells that co-stain for Sox2, Ki-67, or GFAP (right). Values represent mean ± SD and are pooled from three independent experiments. (T–Z) Representative images showing freshly resected normal brain after staining with H&E (T) or for Ki-67 (U) or GFAP (V). (W–Z) Normal brain tissue uninfected (W and X) or infected with ZIKV-Dakar (Y and Z) after 7 d stained for ZIKV (green) and DAPI (blue) with NeuN (red; W and Y) or GFAP (red; X and Z). Images in W–Z are representative of three independent experiments. Significance was analyzed with one-way ANOVA with Tukey’s multiple comparison test (*, P < 0.05; ***, P < 0.001; ****, P < 0.0001). Bars: (A–I and R–Z) 100 µm.