Fig 5. Effects of small molecule inhibitors on pax3 and MyoD induction by IGFs.

Contralateral pairs of forelimbs grafted with IGF-I or IGF-II beads along with small molecule inhibitors, incubated for 24 or 18h and then harvested for in situ hybridization using pax3 or MyoD probes. Black asterisks show positions if IGF beads, red asterisks inhibitor beads. Black arrows show normal expression domains in non-grafted limbs, red arrows induced expression by IGF. Numbers in brackets indicate the number of similar embryos out of total number of grafts. A summary diagram of which molecules inhibited IGF-I and/or IGF-II induction of pax3 and MyoD mRNA is also included.