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. 2017 Sep 18;13(9):e1006637. doi: 10.1371/journal.ppat.1006637

Fig 1. Alcohol intoxication augments susceptibility to CLP and impairs cellular infiltration and bacterial clearance.

Fig 1

A, Enhanced mortality of acute alcohol intoxicated mice after CLP. Alcohol intoxicated and control (saline-challenged) mice were subjected to CLP or a sham procedure. Survival of mice was assessed every 12 h until 10 days after CLP. Results are expressed as a percentage of 20 animals per group from 2 separate experiments. Significance between groups was examined by Wilcoxon rank test. B, Impaired bacterial clearance in alcohol-challenged mice. CFUs were examined in blood, peritoneal lavage or homogenates obtained from livers, lungs, spleens and kidneys of alcohol-challenged and control mice at 6 and 24 h post-CLP. (n = 4-8/group). C, Acute alcohol-intoxicated mice showed reduction in total while blood cell count and neutrophil (PMN) and macrophage accumulation in the peritoneum after CLP, as measured by direct cell counts in peritoneal lavage (PL). (n = 4-8/group). D. Peritoneal cell (neutrophil) viability was not different between alcohol- and saline-challenged mice. A total of 1000 cells were examined in peritoneal fluid of each group was enumerated using trypan blue exclusion assay (n = 4-6/group). *, p<0.05, ***, p<0.001.