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. 2017 Oct 3;12(10):e0185836. doi: 10.1371/journal.pone.0185836

Table 3. Parameters derived from the PN/Ci response curves performed at the end of the experimental period and the chemical and structural leaf traits.

F. ornus p
Parameters C N O3 O3N N O3 O3N
PNmax 25.64 ± 4.40 32.23 ± 4.24 22.73 ± 0.19 22.14 ± 3.65 0.015 0.01 0.09
Vcmax 0.078 ± 0.01 0.067 ± 0.01 0.051 ± 0.00 0.057 ± 0.01 0.742 0.03 0.23
Jmax 106.6 ± 18.44 136.5 ± 13.93 98.32 ± 6.26 91.58 ± 15.58 0.012 0.01 0.04
Γ 48.67 ± 3.68 56.2 ± 18.02 70.65 ± 23.13 52.20 ± 1.68 0.555 0.34 0.18
PNUE 1.9 ± 0.64 1.55 ± 0.28 1.40 ± 0.44 2.19 ± 1.07 0.59 0.86 0.19
NL 1.71 ± 0.09 2.14 ± 0.45 1.26 ± 0.1 1.56 ± 0.32 0.058 0.01 0.71
CL 44.73 ± 0.27 45.31 ± 0.6 44.25 ± 0.45 44.66 ± 0.42 0.094 0.06 0.76
CL/NL 26.15 ± 1.21 21.7 ± 3.97 35.21 ± 2.75 29.49 ± 6.66 0.067 0.01 0.8
LMA 0.013 ± 0.001 0.013 ± 0.001 0.011 ± 0.001 0.012±0.001 0.081 0.003 0.539
Q. ilex p
C N O3 O3N N O3 O3N
PNmax 24.73 ± 3.91 29.9 ± 1.50 23.54 ± 5.41 28.67 ± 3.10 0.003 0.370 0.989
Vcmax 0.076 ± 0.01 0.086 ± 0.009 0.074 ± 0.014 0.10 ± 0.017 0.059 0.498 0.368
Jmax 105.9 ± 12.72 125.8 ± 4.19 102.3 ± 0.99 129.7 ± 8.64 0.000 0.982 0.438
Γ 67.69 ± 1.67 60.06 ± 13.14 67.11 ± 1.92 74.36 ± 12.98 0.972 0.238 0.204
PNUE 3.44 ± 0.97 3.00 ± 0.94 1.84 ± 0.13 2.2 ± 0.54 0.925 0.021 0.372
NL 1.26 ± 0.14 1.44 ± 0.35 1.14 ± 0.07 1.36 ± 0.23 0.158 0.479 0.888
CL 44.43 ± 0.48 45.24 ± 0.37 45.28 ± 0.21 45.55 ± 0.35 0.036 0.026 0.243
CL/NL 35.6 3± 4.03 32.57 ± 7.51 39.79 ± 2.36 34.01 ± 5.45 0.178 0.378 0.663
LMA 0.014 ± 0.001 0.013 ± 0.001 0.015 ± 0.001 0.012 ± 0.001 0.000 0.98 0.01

PNmax, (μmol m-2 s-1) = maximum rate of net photosynthesis; Vcmax, (mol m-2 s-1) = in vivo apparent Rubisco activity; Jmax, (μmol m-2 s-1) = maximum rate of electron transport; Γ, (ppm) = CO2 compensation point; PNUE, photosynthetic nitrogen use efficiency (μmol mol-1 s-1); NL, nitrogen at leaf level (%); CL, carbon concentration at leaf level (%); CL/NL, ratio between carbon and nitrogen at leaf level; Leaf Mass Area (LMA, g cm-2). Data are shown as the mean ± standard deviation (n = 5) for each treatment. On the right panel, results of two-way ANOVA for each parameter are shown. Significant p-value (p < 0.05) are marked in bold; quasi ‘significant’ values (0.1 > p > 0.05) are underlined.