Table 1.

High-occupancy protein targets of acetaminophen 4 in mouse liver as determined by quantitative MS-based proteomics.

Entry	Uniprot ID	Gene name	probe 8 enriched, whole protein	4 competed, whole protein analysis	4 competed, peptide analysis	Competed residue(s), peptide
1	Q9CXT8	Pmpcb	20	20	20	C248*
2	Q9D172	D10Jhu81e	20	19.8	20	C174#
3	P17751	Tpi1	20	10.8	13*, 20#	C71*#
4	Q9DBC0	Selo^	10	20	18*, 20#	U665*#
5	O09131	Gsto1^	20	8.0	1.2*, 20#	C32*#
6	Q9JMH6	Txnrd1^	20	20	5.5*, 6.5#	U612*#
7	P62342	Selt^	20	20	4.3	U49*
8	O09172	Gclm	12	4.9	3.8	C35*
9	Q9CRB3	Urah	20	3.7	2.8*, 6.4#	C35*#
10	Q8BRK8	Prkaa2	5.7	3.1	4.0	C413*
11	Q791V5	Mtch2	6.6	NC	3.4, 5.5	C296*, C56*
12	Q8VHE0	Sec63	8.8	NC	7.5*, 20#	C295*#
13	Q8BUV3	Gphn	9.2	NC	3.9*, 14#	C452*#
14	Q8VCF0	Mavs	20	NC	4.8	C470*
15	Q99LX0	Park7^	11	NC	4.4	C106*
16	Q9DBA6	Tysnd1	5.6	NC	3.6	C106/C110*
17	E9Q3C1	C2cd2	20	NC	3.6	C408*
18	Q9WV55	Vapa	4.3	NC	3.5	C60*
19	O08573	Lgals9	4.8	NC	3.4	C138*
20	P42227	Stat3^	5.8	NC	3.2	C259*
21	Q922Q1	Marc2	4.4	NC	2.4*, 4.0#	C74*#
22	O70252	Hmox2^	20	ND	4.4	C281*
23	Q9JLJ1	Selk^	ND	ND	7.9	U497*
24	Q8VC19	Alas1^	ND	ND	5.6	C108*
25	O08997	Atox1^	ND	ND	4.8	C12/C15*
26	P97821	Ctsc^	ND	ND	3.4	C254/C257*
27	Q9JLC3	Msrb1^	ND	ND	3.4	U95*
28	G5E860	Usp16^	ND	ND	3.3	C204*
29	Q8BH00	Aldh8a1^	1.9	1.5	11*, 12#	C287/289*#
30	Q9JLT4	Txnrd2^	1.8	1.6	3.5*, 4#	U497*#
31	Q9JLJ2	Aldh9a1^	1.7	1	3.9	C288/289*
32	P47738	Aldh2^	1.5	0.9	3.8*, 3.5#	C320-322*#
33	Q9BCZ4	Vimp^	20	20	ND
34	Q9ESW8	Pgpep1^	20	20	ND
35	Q8CDN6	Txnl1^	20	4.7	ND
36	E9Q137	Tex264^	7.1	3.5	ND

^{^}contains conserved functional (seleno)cysteine residue

NC, not competed

ND, not detected

^*Measured by competition with IA alkyne probe (in vitro treatment)

^#Measured by competition with probe 8 (in vivo treatment)