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. 2017 Sep 18;13(9):e1007013. doi: 10.1371/journal.pgen.1007013

Fig 1. Brc1 binding to γH2A is important in pnk1Δ cells.

Fig 1

Tenfold serial dilutions of cells were exposed to the indicated DNA damaging agents. Plates were incubated at 30°C for 3 to 4 days. Doubling times were determined with cells grown in liquid YES media at 32°C. Note that the brc1-T672A and brc1-K710M alleles contain a C-terminal 2GFP tag, which under some conditions can be observed to partially impair Brc1, thus strains with these alleles should be compared to wild type Brc1 tagged with 2GFP (brc1:2GFP).