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. 2017 Sep 18;13(9):e1007013. doi: 10.1371/journal.pgen.1007013

Fig 2. DNA damage checkpoint activation and requirement for checkpoint proteins in pnk1Δ cells.

Fig 2

A) Effects of combining pnk1Δ with rad3Δ or brc1Δ mutations. Tenfold serial dilutions of cells were exposed to the indicated DNA damaging agents. Plates were incubated at 30°C for 3 to 4 days. B) Chk1 also undergoes activating phosphorylation in untreated and HU-treated pnk1Δ cells, unlike in wild type. Cells were incubated with 10 mM HU, 5 μM CPT, or 0.01% MMS for 3 hours. C) Effects of combining pnk1Δ with chk1Δ or brc1Δ mutations. D) Effects of combining pnk1Δ with chk1Δ or cds1Δ.