Fig 8. Increased spontaneous recombination in pnk1Δ cells.

A) Schematic of the non-tandem direct repeat of ade6^- heteroalleles used for measuring spontaneous recombinant frequencies. Conversion types events result in Ade⁺ His^- colonies, whereas deletion types events result in Ade⁺ His⁺ colonies. B) Recombination frequencies (per 10⁴ viable cells ± SD) of the following strains: wild type (1.41 ± 0.57), brc1Δ (1.62 ± 1.16), pnk1Δ (4.78 ± 1.62), brc1Δ pnk1Δ (3.91 ± 2.3). Deletion types and conversion types were determined by replica-plating. Error bars correspond to standard deviations of the means. Asterisk depicts statistically significant differences with wild type and + symbol with pnk1Δ, as determined by two-tailed Student T-test, p-value ≤ 0.05.