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. 2017 Oct 2;216(10):3263–3274. doi: 10.1083/jcb.201611029

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© 2017 Oku et al.

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Figure 2. — Immunoblot analyses detecting cleavage of Vph1-EGFP expressed in mutant strains defective in Atg pathways. The denoted ATG gene–deleted mutant strains (A) or other gene-deletion mutants (B) were cultured at 28°C for 24 h in YEPD medium and subjected to immunoblot analysis as shown in Fig. 1 A.