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. 2017 Oct 2;216(10):3263–3274. doi: 10.1083/jcb.201611029

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© 2017 Oku et al.

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Figure 5. — Efficient transport of LDs into the vacuole in cells after the diauxic shift depends on Vps27 harboring the clathrin-binding motif. (A) Immunoblot analysis of Osw5-EGFP in the designated mutant strains cultured in YEPD medium at 28°C for 16 h. The data for anti–actin antibody are also shown as a loading control. CL; cleaved form; FL, full-length form;. The graph indicates the cleavage rates calculated from three independent experiments. The error bars indicate standard deviation, and the double asterisk indicates statistical significance (**, P < 0.01, t test). (B) The numbers of LDs found inside the vacuoles in EM images of the designated strains (n > 50) were compared. Error bars indicate the standard error. The asterisk indicates the statistical significance of the comparison (*, P < 0.05, t test).