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. 2017 Oct 2;216(10):3323–3338. doi: 10.1083/jcb.201701034

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© 2017 Fiuza et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 1. — PICK1 colocalizes with clathrin in neuronal dendrites. (A) PICK1 colocalizes with clathrin clusters close to synapses. Confocal images of hippocampal neurons transfected with PICK1 shRNA + sh-resistant GFP-PICK1 (green) and dsRed-clathrin (red) and immunostained for the PSD marker Homer (blue). Bar, 2 µm. (B) PICK1 colocalizes with clathrin clusters under super-resolution imaging conditions. Confocal and STED images of hippocampal neurons transfected with PICK1 shRNA + sh-resistant GFP-PICK1 and dsRed-clathrin and immunostained for GFP and dsRed. Bar, 0.5 µm. Graphs show line-scan analyses of PICK1 and clathrin fluorescence intensities of dashed line regions in confocal and STED mode, demonstrating the colocalization of PICK1 and clathrin clusters. Images shown are representative of three independent experiments.