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. 2017 Oct 2;216(10):3275–3290. doi: 10.1083/jcb.201702177

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© 2017 MacDonald and Piper

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Figure 1. — FM4-64 follows a Golgi-independent route from early endosomes to the plasma membrane. FM4-64 efflux measurements (A) and localization of remaining dye (B) from wild-type and vps4Δ cells after 9-min uptake of FM4-64 at 25°C followed by 0- or 15-min chase before the assay. (C) FM4-64 efflux in sec1-1 and sec7-1 ts cells performed at 22°C or 37°C (top), with growth phenotype of ts mutants confirmed (bottom). (D) Colocalization of GFP-Snc1 with FM4-64–labeled endosomes after 10-min uptake assessed in ts mutants at 22°C or 37°C. Bars, 5 µm.