Figure 2.

Various cargoes use an Rcy1-dependent recycling route. (A) Accumulation of FM4-64 at indicated chase times in wild-type and rcy1Δ cells; micrographs were normalized across the series. (B) FM4-64 efflux in indicated mutants. (C) Mup1-GFP localization in wild-type and rcy1Δ cells grown to mid-log phase. (D) Growth assays in wild-type and rcy1Δ cells carrying the trp1 mutation using indicated concentrations of Trp. (E) Wild-type cells expressing either Ste3-GFP or GFP-Snc1 were grown to mid- and late-log phase followed by fluorescence microscopy. (F) Cartography of endocytic pathway: after internalization, cargo enters the Vps4-dependent MVB/degradation pathway or returns from early endosomes or late endosomes to the cell surface via the Golgi/TGN along routes that require Sec7 and Sec1. Cargo such as FM4-64 requires Rcy1 and Sec1, but not Sec7, to return to the plasma membrane. Bars, 5 µm.