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. 2017 Oct 2;216(10):3275–3290. doi: 10.1083/jcb.201702177

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© 2017 MacDonald and Piper

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Figure 7. — The recycling-specific Rag GTPase interactor Ltv1 controls recycling. (A) Ltv1 chimeras from Sc (gray) and Ct (blue) with Rps3 and Rps20 binding sites and phosphomimetic Hrr1 phosphorylation sites shown. (B) FM4-64 efflux of ltv1Δ cells expressing the indicated Ltv1 chimeras. (C) Growth at 30°C and 18°C of the ltv1Δ cells expressing chimeras. (D) Expression levels of HA-tagged chimeras estimated by Western blotting using anti-CPY as a loading control. (E) Wild-type and active nucleotide-bound mutant Rag GTPases tagged with mCherry (RFP) and Ltv1-GFP localized in wild-type cells. (F) Rag GTPases colocalized (arrowheads) with Ltv1-GFP (top) and Ste3-GFP-DUb (bottom) in nhx1Δ cells. (G) Ste3-GFP-Dub and Gtr1-RFP were localized in nhx1Δ ltv1Δ and drs2Δ mutants. Bars, 5 µm.