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Loss of 14-3-3 results in disrupted organization of female meiotic spindles and Msps pole localization. (A–C) Meiotic spindle morphologies in immunostained mature metaphase-I–arrested oocytes depleted of 14-3-3. *, P = 0.013; ***, P < 0.001. n = 170, 174, 16, and 17 (A); n = 17, 17, 21, and 18 (B); n = 49 and 65 (C). Error bars represent 95% confidence intervals. Arrowheads in C indicate concentrations of Msps. (D) Live imaging of mature oocytes expressing GFP–α-tubulin. Bars, 10 µm.
