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. 2017 Oct 2;216(10):3029–3039. doi: 10.1083/jcb.201704120

Figure 3.

Figure 3.

14-3-3 inhibits Ncd microtubule binding. (A) Kinase assay using PKD2 or CAMKIIα, Ncd(58–192) with or without the S96A mutation, and [32P]ATP. (B) Western blot probed by a phospho-S96 Ncd antibody and total protein staining of fractions pulled down by MBP–14-3-3ε after a kinase assay with cold ATP. (C) Microtubule (MT) binding assay. Ncd or Ncd(S96A) was incubated with PKD2 and/or Aurora B (AurB) or without and then mixed with microtubules and GST–14-3-3ε before spinning down. (D) Microtubule binding assay of the Ncd tails with or without MBP–14-3-3ε. P, pellet; S, supernatant.