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. 2017 Oct 2;216(10):3029–3039. doi: 10.1083/jcb.201704120

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© 2017 Beaven et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 4. — Aurora B releases Ncd from inhibition by 14-3-3. (A) Western blot probed by an anti-GFP antibody of pulldown with GST–14-3-3ε from extracts of S2 cells transiently expressing various versions of Ncd-GFP but depleted of the endogenous Ncd. (B) Kinase assay. Coomassie-stained PhosTag gel of the Ncd(58–192) and BSA incubated with kinases. (C) GFP-tagged Ncd tail phosphorylated by kinases (input) and pulled-down fraction with MBP–14-3-3ε. (D) Microtubule (MT) binding assay of differently phosphorylated Ncd tails with or without MBP–14-3-3ε. AurB, Aurora B; P, pellet; S, supernatant.