Fig 4. Elicitor-dependent activation of defence-related promoters in the TZ and DZ of the root.
(a) Quantification of microscopic analysis of Promoter::YFPN constructs in the differentiation zone of 7-days old seedlings following treatment with 100 nM flg22, chi7, AtPep1 or 0.5x MS as control. Images were analysed using Fiji. Bars represent the mean of ≥ 3 images ± SD. (b) Overview of inductive and suppressive responses of Promoter::YFPN constructs in different developmental root zones analysed as described under (a). Numbers in the upper row indicate the sum of significant marker responses in one of the four analysed root zones for a given elicitor, while numbers in the lower row indicate the total sum of significant responses from all markers and all root zones for a given elicitor. Black colour indicates induction, white colour suppression and grey colour non-significant changes.