Table 2. Top 10 metabolites differentially expressed between CRC, PLP and CTRL groups in the discovery set.
Metabolite | CRC | PLP | CTRL | P value | ||||||
---|---|---|---|---|---|---|---|---|---|---|
|
|
|
|
|||||||
N | Mean (SD) | N | Mean (SD) | N | Mean (SD) | CTRL vs PLP | CTRL vs CRC | PLP vs CRC | P for trend | |
Hypoxanthine | 30 | 13.29( 0.49) | 30 | 13.63( 0.67) | 30 | 15.53( 1.33) | <0.01 | <0.01 | 0.06 | <0.01 |
D-Mannose | 30 | 22.44( 0.57) | 30 | 22.16( 0.75) | 30 | 21.62( 0.83) | <0.01 | <0.01 | 0.21 | <0.01 |
DSGEGDFXAEGGGVR* | 30 | 18.87( 1.90) | 30 | 17.50( 1.64) | 30 | 16.81( 2.11) | 0.09 | <0.01 | <0.01 | <0.01 |
Xanthine | 30 | 15.79( 0.51) | 30 | 16.12( 0.42) | 30 | 16.41( 0.70) | 0.11 | <0.01 | 0.01 | <0.01 |
Glutamate | 30 | 22.14( 0.70) | 30 | 22.44( 0.81) | 30 | 22.73( 0.76) | 0.1 | <0.01 | 0.07 | <0.01 |
Tryptophylglutamate | 30 | 12.99( 1.11) | 30 | 13.28( 0.77) | 30 | 13.83( 1.16) | 0.07 | <0.01 | 0.08 | <0.01 |
3-methyl-2-oxobutyrate | 30 | 14.65( 0.38) | 30 | 14.89( 0.33) | 30 | 14.26( 0.40) | <0.01 | <0.01 | <0.01 | <0.01 |
5-oxoproline | 30 | 15.89( 0.36) | 30 | 16.12( 0.59) | 30 | 16.31( 0.63) | 0.12 | <0.01 | 0.08 | <0.01 |
Lactate | 30 | 28.58( 0.64) | 30 | 28.83( 0.50) | 30 | 29.02( 0.71) | 0.09 | <0.01 | 0.09 | <0.01 |
1,3-dipalmitoylglycerol | 30 | 15.09( 0.88) | 30 | 15.63( 0.75) | 30 | 15.75( 0.92) | 0.51 | <0.01 | 0.03 | <0.01 |
Mean level in ng/μL or μg/mL; CRC, colorectal cancer; PLP, adenoma polyp; CTRL, control; SD, standard deviation
Boldface metabolites were selected for further validation.
Small peptide not officially multiplexed by Metabolon based on a standard