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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Mol Cancer Res. 2017 Jul 6;15(10):1331–1340. doi: 10.1158/1541-7786.MCR-17-0180

Figure 4. ISG repression is PR-dependent.

Figure 4

(A). T47D-co cells were transfected with non-silencing (NS) or a pool of four PR siRNAs. 48hr following siRNA transfection, cells were starved for 18hr, followed by 10nM R5020 or vehicle for 6hr. Isolated RNA was analyzed for select genes. Gene values were normalized to an internal control (β-actin). Error bars represent SD between biological triplicates. Asterisks represent statistical significance between the vehicle and R5020-treated groups; p < 0.05, as determined using an unpaired Student’s t-test. This experiment was performed in triplicate, and a representative experiment is shown here. (B). PR knockdown efficiency by siRNA was determined using relative PR levels between the NS and PR siRNA transfected cells. Asterisks represent statistical significance; p < 0.0001. (C). Fold increases in basal (no ligand) ISG expression between PR and NS siRNA transfected cells. Error bars represent the SD for the ratio of two variables using their individual standard deviations, calculated using the Delta method.